Abstract

A multiplex Taqman-based real-time reverse transcription (RT)polymerase chain reaction (PCR) assay was developed to identifypotential severe strains of Citrus tristeza virus (CTV) and separategenotypes that react with the monoclonal antibody MCA13. Three strainspecificprobes were developed using intergene sequences between themajor and minor coat protein genes (CPi) in a multiplex reaction. ProbeCPi-VT3 was designed for VT and T3 genotypes; probe CPi-T36 for T36genotypes; and probe CPi-T36-NS to identify isolates in an outgroupclade of T36-like genotypes mild in California. Total nucleic acidsextracted by chromatography on silica particles, sodium dodecyl sulfatepotassiumacetate, and CTV virion immunocapture all yielded highquality templates for real-time PCR detection of CTV. These assays successfullydifferentiated CTV isolates from California, Florida, and a largepanel of CTV isolates from an international collection maintained inBeltsville, MD. The utility of the assay was validated using field isolatescollected in California and Florida.

Autore Pugliese

• Saponari Maria

Tutti gli autori

• R. K. Yokomi; M. Saponari; P. J. Sieburth

Titolo volume/Rivista

Phytopathology

Anno di pubblicazione

2010

ISSN

0031-949X

ISBN

Non Disponibile

Numero di citazioni Wos

Nessuna citazione

Ultimo Aggiornamento Citazioni

Non Disponibile

Numero di citazioni Scopus

Non Disponibile

Ultimo Aggiornamento Citazioni

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Settori ERC

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Codici ASJC

Non Disponibile