Quantitative analysis of mycotoxins in cereal foods by Collision Cell Fragmentation - High Resolution Mass Spectrometry: performances and comparison with triple stage quadrupole detection.

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Abstract

A liquid chromatography-high-resolution mass spectrometry (LC-HRMS) method for the simultaneousdetermination of aflatoxins (B1, B2, G1, G2), ochratoxin A, deoxynivalenol, zearalenone, T-2 and HT-2 toxinsin wheat flour, barley flour and crisp bread was developed. Mycotoxin fragmentation patterns obtained byhigh-energy collision dissociation (HCD) were investigated to obtain quantitative and confirmatory information(two characteristic masses per mycotoxin) using OrbitrapTM-based high-resolution mass spectrometry.LC-HRMS (full-scan) detection carried out by HCD allows the monitoring of the pseudo-molecular ion andan additional characteristic fragment (for each mycotoxin) with mass accuracy in the range 0.1-3.9 ppm, meetingcurrent European regulatory requirements for LC-MS confirmatory analysis. A sample preparation procedurebased on polymeric solid-phase extraction cartridges was applied, allowing recoveries higher than 74% for ninemycotoxins, with a relative standard deviation lower than 13%. Detection limits in the range 0.5-3.4 mg kg1 wereobtained for three cereal matrices. A critical comparison between the proposed method and a validated methodbased on triple quadrupole mass spectrometry showed similar performance in terms of detection limits, recoveriesand repeatability, and matrix effects. Based on an efficient sample extraction and clean-up, the LC-HCD-HRMSmethod reported here represents a reliable and robust alternative tool for mycotoxin analysis in food matrices ascompared with well-established triple quadrupole-based approaches.

Autore Pugliese

Tutti gli autori

  • Lattanzio V.M.T.; Della Gatta S.; Godula M.; Visconti A.

Titolo volume/Rivista

Food additives & contaminants. Part A. Chemistry, analysis, control, exposure & risk assessment

Anno di pubblicazione

2011

ISSN

1944-0049

ISBN

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