Abstract

The existence of the pyruvate kinase (PK) in pig liver mitochondria was shown by monitoring photometricallythe PK reaction in solubilised mitochondria with either phosphoenolpyruvate (PEP) or ADP used asa substrate. In distinction with the cytosolic isoenzyme, the mitochondrial PK showed a sigmoidal dependenceon either PEP or ADP concentrations. The occurrence of the mitochondrial PK was confirmed byimmunological analysis. Titration with digitonin showed that mPK is restricted to the matrix. PEP additionto mitochondria resulted in reduction of the intramitochondrial NAD(P)+ inhibited by either the nonpenetrantthiol reagent mersalyl or by arsenite, an inhibitor of the pyruvate dehydrogenase complex. Citrate/oxaloacetate appearance outside mitochondria also occurred as result of PEP addition to PLM. Takentogether these findings support a role for PEP itself in triggering fatty acid synthesis via its mitochondrialmetabolism.

Autore Pugliese

• Atlante Anna

Tutti gli autori

• Pizzuto R.; Paventi G.; Atlante A.; Passarella S.

Titolo volume/Rivista

Archives of biochemistry and biophysics

Anno di pubblicazione

2010

ISSN

0003-9861

ISBN

Non Disponibile

Numero di citazioni Wos

Nessuna citazione

Ultimo Aggiornamento Citazioni

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Numero di citazioni Scopus

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Ultimo Aggiornamento Citazioni

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Settori ERC

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Codici ASJC

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