Pru du 2S albumin or Pru du vicilin?
Abstract
A short partial sequence of 28 amino acids is all the information we have so far about the putative allergen 2S albumin from almond. The aim of this work was to analyze this information using mainly bioinformatics tools, in order to verify its rightness. Based on the results reported in the paper describing this allergen from almond, we analyzed the original data of amino acids sequencing through available software. The degree of homology of the almond 12 kDa protein with any other known 2S albumin appears to be much lower than the one reported in the paper that firstly described it. In a publicly available cDNA library we discovered an expressed sequence tag which translation generates a protein that perfectly matches both of the sequencing outputs described in the same paper. A further analysis indicated that the latter protein seems to belong to the vicilin superfamily rather than to the prolamin one. The fact that also vicilins are seed storage proteins known to be highly allergenic would explain the IgE reactivity originally observed. Based on our observations we suggest that the IgE reactive 12 kDa protein from almond currently known as Pru du 25 albumin is in reality the cleaved N-terminal region of a 7S vicilin like protein. (C) 2015 Elsevier Ltd. All rights reserved.
Autore Pugliese
Tutti gli autori
-
Garino C.; De Paolis A.; Coisson J.D.; Arlorio M.
Titolo volume/Rivista
Computational biology and chemistry
Anno di pubblicazione
2015
ISSN
1476-9271
ISBN
Non Disponibile
Numero di citazioni Wos
Nessuna citazione
Ultimo Aggiornamento Citazioni
Non Disponibile
Numero di citazioni Scopus
Non Disponibile
Ultimo Aggiornamento Citazioni
Non Disponibile
Settori ERC
Non Disponibile
Codici ASJC
Non Disponibile
Condividi questo sito sui social