Abstract

A proficiency test for simultaneous determination of up to 11 mycotoxins in maize by using LC-MS(MS) methodology was conducted. Purpose of the study was to evaluate the state of the art on the simultaneous determination of mycotoxins by LC-MS(MS) methodology and to obtain information on currently used methodologies and method-related performances. Laboratories were not obliged to determine all target mycotoxins and were free to report only on those mycotoxins that can be simultaneously determined with their LC-MS(MS) methodology.Participants received two test materials: one maize sample naturally contaminated with aflatoxins B1, B2, G1 and G2, ochratoxin A, deoxynivalenol, HT-2 toxin, zearalenone, fumonisins B1 and B2 and one blank maize sample (for spiking). A common mixed mycotoxins calibrant solution and a spiking solution together with spiking protocol, instructions and a comprehensive questionnaire were also distributed. Forty laboratories from fourteen Countries reported results for some combinations of the analytes. In particular, 25 participants analyzed all target mycotoxins whereas the other participants analyzed between 2-10 mycotoxins. Electrospray ionization was used by all laboratories whereas triple quadrupole and ion trap detector was used by 36 and 4 laboratories, respectively. Sample extract cleanup (IMA, SPE, liquid/liquid extraction) was used by 22 participants whereas 17 laboratories analyzed crude extract without purification and 1 participant analyzed the extract with and without purification before LC-MS(MS) determination. For quantification, 10 participants prepared matrix-assisted calibration curves whereas the other 30 participants prepared calibration curves in pure solvents. To compensate matrix effect, 7 of 30 participants using calibration curves prepared in pure solvents added isotope labeled internal standards to the final extract whereas 1 participant used other metabolites (related to zearalenone or deoxynivalenol) as internal standards. Results on the performance of participating laboratories will be presented with particular reference to z-Scores and percent recovery of the 11 mycotoxins.

Autore Pugliese

• De Girolamo Annalisa , Solfrizzo Michele , Lattanzio Veronica Maria Teresa

Tutti gli autori

• Solfrizzo M.; De Girolamo A.; Lattanzio V.M.T.; Visconti A.; Stroka J.; Alldrick A.J.; van Egmond H.P.

Titolo volume/Rivista

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Anno di pubblicazione

2011

ISSN

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ISBN

978-3-9501610-8-3

Numero di citazioni Wos

Nessuna citazione

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Settori ERC

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