Abstract

Most of the 24 viruses which infect globe artichokeare detrimental to the crop's performance and hamperthe development of a nursery activity in the respect of currentEU legislation. We describe a procedure to sanitize globeartichoke ''Brindisino'' from Artichoke Italian latent virus(AILV) and Artichoke latent virus (ArLV), while preservingits valuable early flowering trait. ArLV was successfullyeliminated by meristem-tip culture,whileAILVwas removedwhen two rounds ofmeristem-tip culture were spaced outwithin vitro thermotherapy. In vivo thermotherapy, followed bymeristem-tip culture, was also successful in producing virusfreematerial but was less efficient in terms of the number ofplants recovered post treatment. Due to the multi-clonalcomposition of the populations at present in cultivation, theselected and sanitised clones were fingerprinted by applyingmicrosatellite and AFLP (amplified fragment length polymorphism)markers.OneAFLP primer combination produced28 informative fragments used to evaluate genetic relatednessamong the clones in study. Our results demonstrates thatAFLP-based molecular fingerprinting enables to verify thetrue to clone correspondence in nurseries, ensure the effectivecorrespondence between the real and the declared identity of aclone, so that to avoid commercial frauds, and might representsa valuable tool for assessing somaclonal variationoccuring during 'in vitro' propagation

Autore Pugliese

• Finetti Sialer Mariella Matilde

Tutti gli autori

• Acquadro A.; Papanice M.; Lanteri S.; Bottalico G.; Portis E.; Campanale A.; Finetti-Sialer M.M.; Mascia T.; Sumerano P. ; Gallitelli D

Titolo volume/Rivista

Plant cell, tissue and organ culture

Anno di pubblicazione

2010

ISSN

1573-5044

ISBN

Non Disponibile

Numero di citazioni Wos

Nessuna citazione

Ultimo Aggiornamento Citazioni

Non Disponibile

Numero di citazioni Scopus

Non Disponibile

Ultimo Aggiornamento Citazioni

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Settori ERC

Non Disponibile

Codici ASJC

Non Disponibile