Abstract

Jasmonic acid (JA) is an important regulator of the plant immune system, playing a central role in the modulation of developmental processes and signalling networks. The JA pathway is an indispensable component of plant resistance to nematodes, and is involved in wound response. Another hormone regulating the systemic wound response in tomato is systemin, an 18-amino-acid peptide signal molecule. Systemin and JA constructively interact in the same signalling pathway, coordinating wound-induced systemic expression of defence-related genes. In the present study we evaluated the expression pattern of selected genes involved in the JA pathway and the susceptibility of different tomato genotypes, including plants expressing different levels of Prosystemin, challenged with the root-knot nematode (RKN) Meloidogyne incognita. Six tomato genotypes: wild type, transformed lines expressing different levels of prosystemin and a homozygous Mi gene resistant tomato, were selected for the infection assay. The seedlings were inoculated four weeks after transplanting with freshly hatched juveniles. Genotypes showing significant differences between measurements were used for gene expression analysis. For this purpose, in vivo plant assays were carried out with tissues collected 5 and 6 days after RKN infection, processed for Real-Time PCR analysis (qRT-PCR). Preliminary data showed that transgenic plants overexpressing Prosystemin had significant less galls and showed milder symptoms, suggesting a Prosystemin role in inducing tolerance to RKN. The genotypes were further analysed in an expression assays with five different genes that appeared involved in the JA pathway. Comparison at two different time points showed that, in the early response, Prosystemin and LOX-D are differentially regulated in the JA pathway. Data at 5 dpi suggested that RKNs suppress the JA pathway in wild type plants and that Prosystemin overexpression could therefore be necessary to induce tolerance to RKNs.

Autore Pugliese

• Finetti Sialer Mariella Matilde , Cillo Fabrizio

Tutti gli autori

• L. Groenenberg; F. Cillo; M. Finetti-Sialer

Titolo volume/Rivista

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Anno di pubblicazione

2015

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ISBN

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Numero di citazioni Wos

Nessuna citazione

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Settori ERC

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Codici ASJC

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