Phylogenetic characterization and ochratoxin A - Fumonisin profile of black Aspergillus isolated from grapes in Argentina.
Abstract
Aspergillus section Nigri populations isolated from seven growing regions from Argentina were characterizedby sequencing in order to identify species responsible for production of ochratoxin A (OTA) and fumonisins(FBs). Sequences of genes encoding calmodulin, ?-tubulin, the second largest subunit of RNA polymerase IIand translation elongation factor 1 alpha were analysed. The phylogenetic analysis showed the presence of sixlineages: A. carbonarius, A. tubingensis, A. niger, A. japonicus, A. homomorphus and A. foetidus grouped in fourmajor clusters. The molecular tools used allowed the identification for the first time of A. homomorphus fromvineyards. OTA production confirmed the importance of A. carbonarius as the main ochratoxigenic speciesisolated and, to a variable degree, of A. niger and A. tubingensis, which were by far the most commonlyoccurring species on grapes in Argentina. The only strains able to produce OTA and fumonisins (B2-B4) belongto the A. niger cluster.
Autore Pugliese
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Chiotta M.L.; Susca A.; Stea G.; Mulè G.; Perrone G.; Logrieco A.; Chulze S.N.
Titolo volume/Rivista
International journal of food microbiology
Anno di pubblicazione
2011
ISSN
0168-1605
ISBN
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Codici ASJC
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