Abstract

High-level expression of the GUP1 gene in Saccharomyces cerevisiae resulted in the formation of proliferatedstructures, which hosted endoplasmic reticulum (ER), Golgi and itinerant proteins. The GUP1 over-expressionenhanced ER biogenesis, as shown by the coordinated increased transcription rate of genes involved in both ERand Golgi metabolism and in phospholipids biosynthesis. The formation of Gup1-induced proliferation revealedthat it depended on an intact unfolded protein response, because their assembly was reported to be lethal toyeast strains unable to initiate the UPR (Unfolded Protein Response) pathway. GUP1 over-expression affectedglobal ER and Golgi structure and resulted in the biogenesis of novel membrane arrays with Golgi and ER hybridcomposition. In fact, a number of ER and Golgi resident proteins together with itinerant proteins that normallycycle between ER and Golgi, were localized in the proliferated stacked membranes. The described assembling ofnovel membrane structures was affected by the functionality of the Gup1 O-acyltransferase domain, whichregulates the Gup1 protein role as remodelase in the glycosylphosphatidylinositol (GPI) anchored proteinsbiosynthesis. To our knowledge, we presented the first evidence of sub cellular modifications in response overexpressionof a GPI-anchor remodelase in S. cerevisiae.

Autore Pugliese

• Grieco Francesco , Bleve Gianluca

Tutti gli autori

• Bleve G.; Di Sansebastiano G.; Grieco F.

Titolo volume/Rivista

Biochimica et biophysica acta. Biomembranes

Anno di pubblicazione

2011

ISSN

0005-2736

ISBN

Non Disponibile

Numero di citazioni Wos

Nessuna citazione

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Numero di citazioni Scopus

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Settori ERC

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Codici ASJC

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