Abstract

Premise of the study: Pinus pinea is one of the few widespread plant species that are also genetically depauperate. It is also an important commercial species with high market value seeds. A deeper knowledge of the existing population genetic variation was needed.Methods and Results: Twelve nuclear microsatellites were isolated from genomic and cDNA sequences and screened for variability in 729 individuals from 33 natural populations. Low level of genetic variability was confirmed with average expected heterozygosity of 0.11. Hardy-Weinberg equilibrium expectations were not met in only similar to 10% of the possible locus/population combinations. All loci were in linkage equilibrium, and the frequency of alleles was very low (<= 1% in 332 out of 396 locus/population combinations). Nine out of the 12 microsatellites were successfully transferred to P. halepensis.Conclusions: Despite low polymorphism, these new markers will be useful to resolve population structure and hold potential for seed origin identification and traceability.

Autore Pugliese

• Vendramin Giovanni Giuseppe

Tutti gli autori

• F. Pinzauti; F. Sebastiani; K.B. Budde; B. Fady; S.C. González-Martínez; G.G. Vendramin

Titolo volume/Rivista

American journal of botany

Anno di pubblicazione

2012

ISSN

0002-9122

ISBN

Non Disponibile

Numero di citazioni Wos

Nessuna citazione

Ultimo Aggiornamento Citazioni

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Numero di citazioni Scopus

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Ultimo Aggiornamento Citazioni

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Settori ERC

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Codici ASJC

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