New nematodes associated to Rhynchophorus ferrugineus (Coleoptera: Curculionidae): preliminary description.
Abstract
Several nemamdes, juveniles and adults, were found dissecting some Rhynchophorus ferrugineus pupae and adults from infested Phoenix canariensis exemplar in Bari (Italy). Insect was intact externally but inner tissues were completely liquefied. Nematodes were collected using the water trap method and total DNA was extracted from each individual. The 18s rDNA, the ITS containing region and the mitochondrial cytochrome oxidase I (COI) were amplified md sequenced. ITS-RFLP analysis were also obtained. BLAST search revealed that nucleotides sequences are similar (93%) to Koerneria sp. R81982 (Nematoda: Diplogastidae). Nematodes belonging to Diplogastridae are commonly associated with kts, with different types of association depending on diplogastrid genera. Koerneria spp. are frequently associated with stag and dung hties. Characterization studies are now still in progress for the species identification. Our future purpose is to clarify the kind of association between this specie and the Red Weevil and the eventual role as natural control agent.
Autore Pugliese
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Oreste M.; De Luca F.; Fanelli E.; Troccoli A.; Tarasco E.
Titolo volume/Rivista
IOBC-WPRS Bulletin
Anno di pubblicazione
2013
ISSN
1027-3115
ISBN
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Codici ASJC
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