Abstract

Nm23-H1 is one of the most interesting candidate genes for a relevant role in Neuroblastoma pathogenesis. H-Prune is the most characterized Nm23-H1 binding partner, and its overexpression has been shown in different human cancers. Our study focuses on the role of the Nm23-H1/h-Prune protein complex in Neuroblastoma. Using NMR spectroscopy, we performed a conformational analysis of the h-Prune C-terminal to identify the amino acids involved in the interaction with Nm23-H1. We developed a competitive permeable peptide (CPP) to impair the formation of the Nm23-H1/h-Prune complex and demonstrated that CPP causes impairment of cell motility, substantial impairment of tumor growth and metastases formation. Meta-analysis performed on three Neuroblastoma cohorts showed Nm23-H1 as the gene highly associated to Neuroblastoma aggressiveness. We also identified two other proteins (PTPRA and TRIM22) with expression levels significantly affected by CPP. These data suggest a new avenue for potential clinical application of CPP in Neuroblastoma treatment.

Autore Pugliese

• Saviano Michele

Tutti gli autori

• Carotenuto M ; Pedone E ; Diana D ; de Antonellis P ; Dzeroski S ; Marino N ; Navas L ; Di Dato V ; Scoppettuolo MN ; Cimmino F ; Correale S ; Pirone L ; Monti SM ; Bruder E ; Zenko B ; Slavkov I ; Pastorino F ; Ponzoni M ; Schulte JH ; Schramm A ; Eggert A; Westermann F ; Arrigoni G ; Accordi B ; Basso G ; Saviano M ; Fattorusso R ; Zollo M

Titolo volume/Rivista

Scientific reports

Anno di pubblicazione

2013

ISSN

2045-2322

ISBN

Non Disponibile

Numero di citazioni Wos

Nessuna citazione

Ultimo Aggiornamento Citazioni

Non Disponibile

Numero di citazioni Scopus

Non Disponibile

Ultimo Aggiornamento Citazioni

Non Disponibile

Settori ERC

Non Disponibile

Codici ASJC

Non Disponibile