Abstract

Phytoparasitic nematodes can cause severe yield losses to numerous vegetables therefore it is necessary their control. To evaluate the efficacy of two essential oils (EOs) isolated from Thymus vulgaris and Cinnamomum camphora in the control of the potato cyst nematode Globodera rostochiensis an in vitro experiment (hatching test) was carried out.Batches of 50 G. rostochiensis cysts, of similar size, extracted by the Fenwick can from infested soil, were placed in Eppendorf tubes (2mL) in 1 mL of distilled water before treatments with EOs. The two EOs solutions (10% ethanol, v/v) in appropriate amount were diluted in distilled water containing 0.3% polysorbate surfactant 20 (Tween-20) (v/v) to obtain 4, 8 and 16 ?L mL-1 solutions. The EOs were then used to treat G. rostochiensis cysts adding to each Eppendorf tube 1 mL of each solution. Then, tubes were closed and cysts exposed to the EOs treatment for 48 hrs. Untreated cysts were used as control. Four replications for each treatment were considered. After treatments cysts were removed from the Eos solutions, rinsed in distilled water and put in 2 cm diam sieves (215 ?m aperture) placed in 3.5 cm diam Petri dishes. In the hatching test potato root leachate was used as natural hatching agent and the test was carried out in a growth cabinet at 21 °C. Emerged juveniles were removed and counted every week over a 6 week period. The hatching agent was renewed at each observation time. Numbers of second stage juveniles emerging every week were expressed as cumulative percentages of the total egg content of the cysts.Percentage hatch of G. rostochiensis eggs was significantly reduced (P < 0.05) by the different concentrations of both Eos, although significantly higher eggs mortality was observed in cysts immersed in thyme oil. Our results suggest that essential oils of Thymus and Cinnamon could be favourably considered in a sustainable control of the potato cyst nematode, although further studies are needed.Acknowledgement: This study was supported by the VEGA scientific grant agency, grant No. 2/0079/13 and by the Agreement between CNR (Italy) and ASM (R. Moldova) for the years 2015-16 for the project "Agro technical and biological methods for a sustainable control of quarantine cyst nematodes in Italy and in R. Moldova

Autore Pugliese

• Sasanelli Nicola

Tutti gli autori

• Renco M.; Cerevková A.; Sasanelli N.; Toderas I.

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Anno di pubblicazione

2015

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