Abstract

Subsistence farmers are exposed to a range of mycotoxins. This study applied novel urinary multi-mycotoxinLC-MS/MS methods to determine multiple exposure biomarkers in the high oesophageal cancerregion, Transkei, South Africa. Fifty-three female participants donated part of their maize-based eveningmeal and first void morning urine, which was analysed both with sample clean-up (single and multi-biomarker)and by a 'dilute-and-shoot' multi-biomarker method. Results were corrected for recovery withLOD for not detected. A single biomarker method detected fumonisin B1 (FB1) (87% incidence; mean ± standarddeviation 0.342 ± 0.466 ng/mg creatinine) and deoxynivalenol (100%; mean 20.4 ± 49.4 ng/mg creatinine)after hydrolysis with b-glucuronidase. The multi-biomarker 'dilute-and-shoot' method indicateddeoxynivalenol-15-glucuronide was predominantly present. A multi-biomarker method with b-glucuronidaseand immunoaffinity clean-up determined zearalenone (100%; 0.529 ± 1.60 ng/mg creatinine), FB1(96%; 1.52 ± 2.17 ng/mg creatinine), a-zearalenol (92%; 0.614 ± 1.91 ng/mg creatinine), deoxynivalenol(87%; 11.3 ± 27.1 ng/mg creatinine), b-zearalenol (75%; 0.702 ± 2.95 ng/mg creatinine) and ochratoxin A(98%; 0.041 ± 0.086 ng/mg creatinine). These demonstrate the value of multi-biomarker methods inmeasuring exposures in populations exposed to multiple mycotoxins. This is the first finding of urinarydeoxynivalenol, zearalenone, their conjugates, ochratoxin A and zearalenols in Transkei.

Autore Pugliese

• Gambacorta Lucia , Solfrizzo Michele

Tutti gli autori

• G.S. Shephard ; H.-M. Burger ; L. Gambacorta ; Y.Y. Gong ; R. Krska ;J.P. Rheeder ; M. Solfrizzo ; C. Srey ; M. Sulyok ; A. Visconti ; B. Warth ;L. van der Westhuizen

Titolo volume/Rivista

Food and chemical toxicology

Anno di pubblicazione

2013

ISSN

0278-6915

ISBN

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Numero di citazioni Wos

Nessuna citazione

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Numero di citazioni Scopus

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Settori ERC

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