Multidisciplinary approach for characterisation and Valorisation of legume and cereal genetic resources.

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Abstract

During the last two decades, our research activities have been focused on four different topics: 1)evaluation and characterization of wild and cultivated genetic resources using multidisciplinaryapproaches; 2) selection of genotypes having useful genetic, agronomic, quality and nutritionaltraits; 3) production of useful pre-breeding lines; 4) support to local communities to promote thecultivation in the traditional areas of the local varieties (on-farm conservation) with interestingagronomic performance and good nutritional and technological traits. The target seed componentsinvestigated are:o for legumes: anti-nutritionals, toxic factors, non-protein amino acids, and protein fractionswith high functional value (enzymes, enzyme inhibitors);o for cereals: gluten and waxy proteins associated to nutritional, functional and technologicalaspects of flour and/or semolina; macro and micro elements in whole and refined flour.Genetic variation at wheat and bean protein loci, seed storage protein composition, technologicaland nutritional quality, agronomic performances, aptitude to industrial processing are investigatedby several biochemical techniques, such as, one and two-dimensional gel electrophoresis (1D- and2D-PAGE), capillary electrophoresis (CE), reversed phase high performance liquidchromatography (RP-HPLC), gas-chromatography (GC), spectrophotometric dosages, molecularmarkers and proteomic approaches.Characterisation and valorisation of legume and cereal genetic resources allow to acquire usefulinformation to plain appropriate actions of safeguard and management of germplasm collections,as well as to support the request of quality marks for specific local varieties.Moreover, the identification of segments of genetic resources carrying out useful traits, is essentialto direct breeder programs during the pre-breeding selection. Case-studies of utilisation andvalorisation of genetic resources will be illustrated, along with the participation to research projectssupported by private and public institutions.

Autore Pugliese

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  • Piergiovanni A.R.; Lioi L.; Bisignano V.; Urbano M.; Margiotta B.

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Anno di pubblicazione

2014

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