Morphological and molecular characterization of a Steinernema carpocapsae (Nematoda Steinernematidae) strain isolated in Veneto region (Italy)
Abstract
A new strain of Steinernema carpocapsae (Weiser, 1955) was isolated from soil collected in a lagoon plain in Veneto region (North-East Italy). This new strain was named ItS-CAO1. Molecular and morphological analyses were performed. The ITS region and the 18S rRNA gene were amplified and sequenced. The ITS products were then digested with six restriction enzymes in order to unequivocally identify this species. Nematode virulence was tested against last instar of Galleria mellonella (L.) using different laboratory assays. Insect mortality of this new strain is very high in penetration (100%) and sand column assay (93.3%) and the percentage of penetrating infective juveniles was 57.6 and 42.9, respectively. Larval mortality in one-on-one quality assay was 50% and in exposure time assay it was 50% at 19 minutes. With the results of infectivity assays we can evaluate the possibility to use this new strain in biological control programs.
Autore Pugliese
Tutti gli autori
-
Torrini G.; Landi S.; Benvenuti C.; De Luca F.; Fanelli E.; Troccoli A .; Tarasco E.; Bazzoffi P.; Roversi P.F.
Titolo volume/Rivista
Redia
Anno di pubblicazione
2014
ISSN
0370-4327
ISBN
Non Disponibile
Numero di citazioni Wos
Nessuna citazione
Ultimo Aggiornamento Citazioni
Non Disponibile
Numero di citazioni Scopus
Non Disponibile
Ultimo Aggiornamento Citazioni
Non Disponibile
Settori ERC
Non Disponibile
Codici ASJC
Non Disponibile
Condividi questo sito sui social