Molecular variability and phylogeny of Schistonchus caprifici (Gasperrini, 1864) Cobb, 1927 (Nematoda: Aphelenchoididae) from Italy and Spain.

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Abstract

Molecular characterisation of four Schistonchus caprifici populations (two from Spain and two from Italy), using the partial18S, the D2-D3 expansion segments of 28S of ribosomal DNA (rDNA) and the partial mitochondrial gene for cytochrome c oxidaseI (mtCOI), is provided. Amplicons from partial mtCOI were analysed using ‘Single Strand Conformation Polymorphism’ (SSCP)analysis in order to rapidly screen for genetic (haplotypic) variability. SSCP analysis of mtCOI revealed a close relationship betweenthe Spanish and Italian populations with low intra-population variability and only two haplotypes were detected.Mitochondrial COI andribosomal genes were analysed using Maximum Likelihood and Bayesian inference to reconstruct phylogenetic relationships withinS. caprifici and other members of the family Aphelenchoididae. The phylogenetic analysis based on mtCOI showed no geographicvariability among Italian, Spanish and Turkish populations. The results of phylogenetic analyses based on the D2-D3 expansionsegments of 28S rDNA region and the partial 18S rDNA genes revealed a closer phylogenetic relationship between S. aureus,S. laevigatus, S. virens and S. centerae, whilst S. caprifici and S. guangzhouensis clustered separately. The phylogeny of the genusSchistonchus was well related to some morphological characters, such as position of excretory pore, presence/absence of labial discand number and position of caudal papillae. The monophyly of the genus Schistonchus was rejected by the Shimodaira-Hasegawa testbased on tree topologies

Autore Pugliese

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  • De Luca F.; Castillo P.; Troccoli A.; Vovlas N.; Landa B.B.; Palomares-Rius J.E.

Titolo volume/Rivista

Nematology

Anno di pubblicazione

2010

ISSN

1388-5545

ISBN

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Numero di citazioni Wos

Nessuna citazione

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Settori ERC

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Codici ASJC

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