Abstract

The occurrence of a male-less population of Sphaeronema alni parasitising chestnut (Castanea sativa) roots and inducinga stelar syncytium is reported for the first time in Pola de Somiedo (Oviedo province), Spain.Morphometric and molecular characters ofthe Spanish population matched those of a topotype population from Russia. SEM observations showed swollen females having the firstlip annulus wider than the second and appearing as a cap-like, circumoral elevation. The second-stage juveniles, having a single band inthe lateral fields, were characterised by a non-annulated dome-shaped lip region derived from the fusion of the oral disc with all the lipsectors and lip annuli, and showing slit-like amphidial apertures and an oval prestoma. The sequences of the D2-D3 expansion segmentsof 28S rRNA, partial 18S rRNA and ITS rRNA gene for the Spanish and topotype populations of S. alni were congruent and matchedthose deposited in GenBank for another population from Germany, thereby confirming their conspecificity. A PCR-RFLP profile ofD2-D3 of 28S rRNA for identification of this species was also provided. The phylogenetic relationships between S. alni populationsand representatives of the suborder Criconematina, as inferred from analysis of partial 18S rRNA and D2-D3 of 28S gene sequencesobtained in this and previous studies, indicated that S. alni formed a basal clade on the majority consensus Bayesian phylogenetic trees,standing together with Meloidoderita sp. or alone. These findings provide additional evidence of the need to clarify the position ofSphaeronema within Criconematina and its relationships with representatives of Tylenchulinae.

Autore Pugliese

• Troccoli Alberto

Tutti gli autori

• Palomares-Rius J.E.; Vovlas N.; Subbotin S.A.; Troccoli A.; Cantalapiedra-Navarrete C.; Liébanas G.; Chizhov V. N.; Landa B. B.; Castillo P.

Titolo volume/Rivista

Nematology

Anno di pubblicazione

2010

ISSN

1388-5545

ISBN

Non Disponibile

Numero di citazioni Wos

Nessuna citazione

Ultimo Aggiornamento Citazioni

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Numero di citazioni Scopus

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Settori ERC

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Codici ASJC

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