Abstract

A two-year study on the pathosystem F. verticillioides-maizewas conducted speculating on both in vitro and in planta perspectives.The former studied the effects of temperature (T) andwater activity (aw) on fumonisin B (FBs) production and expressionof FUM2 and FUM21 genes in ITEM 10027 and 1744 F. verticillioidesstrains grown up to 21 days. The latter monitoredwhich genes were differentially expressed in resistant and susceptiblemaize lines after 2-3 days infection by ITEM 1744. The invitro study showed that ITEM 10027 was the highest FBs producer,with predominance of FB1. The maximum level of FB wasregistered after 21 days in both strains. FUM2 and FUM21 wereexpressed in all studied conditions, with a 10X difference betweenthe former and the latter. The peak of transcription levelwas reached after 14 days of incubation for both FUM genes. Nodata were collected on cultures grown at fixed aw=0.900 becausethe fungus did not grow at all temperatures tested till 21 days ofincubation. The in planta study showed that resistant lines testedwere poorly infected by ITEM 1744. Genes differentially expressedwere divided into 11 functional categories and nearly10% was assigned to the class "cell rescue, defence and virulence".Most of the pathogenesis-related genes were differentiallyactivated after fungal infection in relation to the resistance levelof maize genotypes. In the resistant kernels, defence-relatedgenes provided basal protection against the fungus, while in thesusceptible kernels, the same genes were induced specifically afterpathogen attack.

Autore Pugliese

• Mule' Giuseppina , Susca Antonia

Tutti gli autori

• G. Mulè; I. Lazzaro; A. Susca; A. Ritieni; A. Lanubile; J. Bernardi; A. Marocco; P. Battilani

Titolo volume/Rivista

Journal of plant pathology

Anno di pubblicazione

2011

ISSN

1125-4653

ISBN

Non Disponibile

Numero di citazioni Wos

Nessuna citazione

Ultimo Aggiornamento Citazioni

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Numero di citazioni Scopus

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Ultimo Aggiornamento Citazioni

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Settori ERC

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Codici ASJC

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