Abstract

The diversity of indigenous Oenococcus oeni strains was investigated by molecular and biochemical characterization of isolates from Malvasia Nera wine, an economically important red wine of the Salento Region (Apulia, Italy), during spontaneous malolactic fermentation (MLF). A total of 82 isolates of this species, identified by species-specific PCR and 16S rDNA sequence analysis, were molecularly characterized by the Amplified Fragment Length Polymorphism (AFLP) technique. Three main groups resulted by clustering analysis and showed intraspecific homology higher than 50% and a total of seven subgroups, with similarity values ranged from 80 to 98%, were obtained within these groups. Enzymatic activities, such as esterase, ²-glucosidase, protease, and the consumption rate of L-malic acid, citric acid, acetaldehyde and arginine were assessed in the representative strains according to AFLP analysis. The results displayed different enzymatic activities and consumption rates of tested metabolites among the strains. No correlation between molecular and biochemical data was observed. The evidence of biochemical variability observed among ‘Malvasia Nera’ strains demonstrated that the wine aroma can be modulated depending on the strains involved in the MLF. Hence, the heterogeneity existing within natural O. oeni populations represents an interesting ecological source that can be useful for technological purposes.

Autore Pugliese

• Logrieco Antonio Francesco , Cappello Maria Stella

Tutti gli autori

• Cappello M.S; Zapparoli G.; Stefani D.; Logrieco A.

Titolo volume/Rivista

Systematic and applied microbiology

Anno di pubblicazione

2010

ISSN

0723-2020

ISBN

Non Disponibile

Numero di citazioni Wos

Nessuna citazione

Ultimo Aggiornamento Citazioni

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Numero di citazioni Scopus

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Ultimo Aggiornamento Citazioni

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Settori ERC

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Codici ASJC

Non Disponibile