Molecular analysis of helicotylenchus cf. Dlgonlcus from south of iran based on sequence of the 28s ribosomal dna

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Abstract

Helicotylenchus is a cosmopolitan genus including some important plant parasiticnematodes causing damage in agricultural and horticultural crops. During a soil sampling ofalmond (Prunus) orchards in Baft region of Kerman province (South East of Iran), a species ofHelicotylenchus was extracted from rizosphere of almonds and identified according tomorphological and morphometric characters as H. digonicus. In addition, the D2D3 segment of 28srDNA was amplified using specific primers and sequenced. The Nblast result showed that studiedpopulation has 8 nucleotides differences with Italian population of H. digonicus (DQ328758; 99%identity). Phylogenetic analysis using Maximum Likelihood places this population close to anundescribed population of Helicotylenchus (HM014303) and H. digonicus. Genetic pairwisedistances showed low variation (0.002) with a population of H. digonicus (HM014241) from SouthAfrica.

Autore Pugliese

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  • Nadi M.; Shokoohi E.; Troccoli A .

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Anno di pubblicazione

2013

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