Abstract

The wheat proteins soluble in chloroform-methanol mixtures are associated with severalkinds of food allergies. A separation method based on capillary zone electrophoresis (CZE) with UVdetection was developed for the analysis of these mixtures. An acidic phosphoric acid/-alanine(pH 2.5) buffer containing HPMC, urea and acetonitrile was used for the separation. The capillaryelectrophoresis (CE) was able to complete the analysis in six minutes. The electrophoregrams ofextracts of both durum and common wheat commercial cultivars were compared. The registeredcultivar (cv.) Kamut® was included as a representative of rustic cereal species. A different number ofpeaks were detected in the profile relative to the tetraploid and exaploid analyzed cultivars. Threemain peaks were observed for all tetraploid cultivars, while four peaks were detected for the commonwheat cultivars. The peak corresponding to the -amylase inhibitor type III was identified in thecommon wheat electrophoregram. The possibility of quantitative determination of this inhibitor hasbeen investigated.

Autore Pugliese

• Piergiovanni Angela Rosa

Tutti gli autori

• A.R. Piergiovanni

Titolo volume/Rivista

Separations

Anno di pubblicazione

2016

ISSN

2297-8739

ISBN

Non Disponibile

Numero di citazioni Wos

Nessuna citazione

Ultimo Aggiornamento Citazioni

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Numero di citazioni Scopus

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Ultimo Aggiornamento Citazioni

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Settori ERC

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Codici ASJC

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