Abstract

The development of fast, reliable and culture-independent molecular tools to detect bacteria producingbiogenic amines deserves the attention of research and ultimately of the food industry in order to protectconsumers' health. Here we present the application of a simple, low-cost, fast and sensitive method toperform microdroplet-based multiplex PCR, directly on a food matrix, for the simultaneous detection ofbacterial genes involved in biogenic amine biosynthesis. After inoculating wine with Lactobacillus brevisIOEB 9809, cell lysis and DNA amplification are performed in one single step, without preliminary nucleicacid extraction or purification treatments. The assay is performed in about 30 min, requiring 150 nL ofstarting sample and it enables the detection of down to 15 bacterial cells. With respect to traditionalculture techniques, the speed, the simplicity and the cheapness of this procedure allow an effectivemonitoring of microbial cells during food-making and processing.

Autore Pugliese

• Grieco Francesco , Mita Giovanni

Tutti gli autori

• Sciancalepore A.G.; Mele E.; Arcadio V; Reddavide F.; Grieco F.; Spano G.; Lucas P.; Mita G.; Pisignano D.

Titolo volume/Rivista

Food microbiology

Anno di pubblicazione

2013

ISSN

0740-0020

ISBN

Non Disponibile

Numero di citazioni Wos

Nessuna citazione

Ultimo Aggiornamento Citazioni

Non Disponibile

Numero di citazioni Scopus

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Ultimo Aggiornamento Citazioni

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Settori ERC

Non Disponibile

Codici ASJC

Non Disponibile