Abstract

Microbial dynamics and enzymatic activities of activated sludge processes are not completelyunderstood yet. A better understanding about the biology is indispensable for further processoptimization. Since proteins play a key role as catalysts in sludge processes, a protocol forprotein extraction and analysis by 2-D PAGE was established. It is based on phenol extractionof alkaline extracts and on a subsequent precipitation with ammonium sulphate. 2-D proteinpatterns obtained from different sludges collected from membrane bioreactors showed -besides common spots - significant differences. Selected proteins were identified with nano-HPLC-ESI-MS/MS. All membrane biological reactor (MBR) sludge samples investigated inthis study contained elastase 3A, which implies that this human serine protease is a significantconstituent of municipal wastewater. Although the identification of proteins fromammonia-oxidizing bacterium Nitrosomonas europaea was expected, the detection of a proteinwith homology to the marine bacterium Saprospira grandis in MBR1 was surprising.

Autore Pugliese

• Pollice Alfieri

Tutti gli autori

• Kuhn R.; Benndorf D.; Rapp E.; Reichl U.; Palese L.L.; Pollice A.

Titolo volume/Rivista

Proteomics

Anno di pubblicazione

2011

ISSN

1615-9853

ISBN

Non Disponibile

Numero di citazioni Wos

Nessuna citazione

Ultimo Aggiornamento Citazioni

Non Disponibile

Numero di citazioni Scopus

Non Disponibile

Ultimo Aggiornamento Citazioni

Non Disponibile

Settori ERC

Non Disponibile

Codici ASJC

Non Disponibile