Abstract

Oleosin, caleosin and steroleosin, are normally expressed indeveloping seed cells and are targeted to oil bodies. In the present work,the cDNA of each gene, tagged with fluorescent proteins, was transientlyexpressed into tobacco protoplasts, and the fluorescent patterns observedby confocal laser scanning microscopy. Our results indicated cleardifferences in the endocellular localisation of the three proteins. Oleosinand caleosin both share a common structure consisting of a centralhydrophobic domain flanked by two hydrophilic domains and were correctlytargeted to LDs, whereas steroleosin, characterised by an N-terminal oilbody anchoring domain, was mainly retained in the endoplasmic reticulum (ER). Protoplasts fractionation on sucrose gradients indicated that both oleosinand caleosin-GFP peaked at different fractions than where steroleosin-GFPor the ER marker BiP, were recovered. Chemical analysis confirmed thepresence of triacylglycerols in one of the fractions where oleosin-GFP wasrecovered. Finally, only oleosin- and caleosin-GFP were able toreconstitute artificial oil bodies in the presence of triacylglycerols andphospholipids. Taken together our results pointed for the first time thatleaf LDs can be separated by the ER and both oleosin or caleosin areselectively targeted due to the existence of selective mechanismscontrolling protein association with these organelles.

Autore Pugliese

• Santino Angelo , Poltronieri Palmiro

Tutti gli autori

• De Domenico S.; Bonsegna S.; Lenucci M.S.; Poltronieri P.; Di Sansebastiano G.P.; Santino A.

Titolo volume/Rivista

Journal of integrative plant biology

Anno di pubblicazione

2011

ISSN

1672-9072

ISBN

Non Disponibile

Numero di citazioni Wos

Nessuna citazione

Ultimo Aggiornamento Citazioni

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Numero di citazioni Scopus

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Ultimo Aggiornamento Citazioni

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Settori ERC

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Codici ASJC

Non Disponibile