Abstract

The carnitine/acylcarnitine transporter is atransport system whose function is essential for the mitochondrialb-oxidation of fatty acids. Here, the presence ofcarnitine/acylcarnitine carrier (CACT) in nervous tissueand its sub-cellular localization in dorsal root ganglia(DRG) neurons have been investigated. Western blot analysisusing a polyclonal anti-CACT antibody produced inour laboratory revealed the presence of CACT in all thenervous tissue extracts analyzed. Confocal microscopyexperiments performed on fixed and permeabilized DRGneurons co-stained with the anti-CACT antibody and themitochondrial marker MitoTracker Red clearly showed amitochondrial localization for the carnitine/acylcarnitinetransporter. The transport activity of CACT from DRGextracts reconstituted into liposomes was about 50 % inrespect to liver extracts. The experimental data herereported represent the first direct evidence of the expressionof the carnitine/acylcarnitine transporter in sensoryneurons, thus supporting the existence of the b-oxidationpathway in these cells

Autore Pugliese

• Tonazzi Annamaria

Tutti gli autori

• A. Tonazzi; C. Mantovani; M. Colella; G. Terenghi; C. Indiveri

Titolo volume/Rivista

Neurochemical research

Anno di pubblicazione

2013

ISSN

0364-3190

ISBN

Non Disponibile

Numero di citazioni Wos

Nessuna citazione

Ultimo Aggiornamento Citazioni

Non Disponibile

Numero di citazioni Scopus

Non Disponibile

Ultimo Aggiornamento Citazioni

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Settori ERC

Non Disponibile

Codici ASJC

Non Disponibile