Knock-out of metacaspase and/or cytochrome c results in the activation of a ROS-independent acetic acid-induced programmed cell death pathway in yeast

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Abstract

To gain further insight into yeast acetic acid-induced programmed cell death (AA-PCD) we analyzed the effects of the antioxidant N-acetyl-L-cysteine (NAC) on cell viability, hydrogen peroxide (H2O2)production, DNA fragmentation, cytochrome c (cyt c) release and caspase-like activation in wild type (wt) and metacaspase and/or cyt c-lacking cells. We found that NAC prevents AA-PCD in wt cells, by scavenging H2O2 and by inhibiting both cyt c release and caspase-like activation. This shows the occurrence of a reactive oxygen species (ROS)-dependent AA-PCD. Contrarily no NAC dependentchange in AA-PCD of mutant cells was detectable, showing that a ROS-independent AA-PCD can also occur.

Autore Pugliese

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  • Guaragnella N.; Passarella S.; Marra E.; Giannattasio S.

Titolo volume/Rivista

FEBS letters

Anno di pubblicazione

2010

ISSN

0014-5793

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