Abstract

Squarebop I bacteriorhodopsin is a light-activated proton pump present in the membranes of the archeonHaloquadratum walsbyi, a square-shaped organism representing 50-60% of microbial population in thecrystallizer ponds of the coastal salterns.Here we describe: (1) the operating mode of a bioreactor designed to concentrate the saltern biomassthrough a microfiltration process based on polyethersulfone hollow fibers; (2) the isolation of SquarebopI bacteriorhodopsin from solubilized biomass by means of a single chromatographic step; (3) tightlybound lipids to the isolated and purified protein as revealed by MALDI-TOF/MS analysis; (4) the photoactivityof Squarebop I bacteriorhodopsin isolated from environmental samples by flash spectroscopy.Yield of the isolation process is 150 lg of Squarebop I bacteriorhodopsin from 1 l of 25-fold concentratedbiomass. The possibility of using the concentrated biomass of salterns, as renewable resourcefor the isolation of functional bacteriorhodopsin and possibly other valuable bioproducts, is brieflydiscussed.

Autore Pugliese

• Milano Francesco , Pollice Alfieri , Laera Giuseppe

Tutti gli autori

• Lobasso S.; Lopalco P.; Angelini R.; Pollice A.; Laera G.; Milano F.; Agostiano A.; Corcelli A.

Titolo volume/Rivista

Protein expression and purification

Anno di pubblicazione

2012

ISSN

1046-5928

ISBN

Non Disponibile

Numero di citazioni Wos

Nessuna citazione

Ultimo Aggiornamento Citazioni

Non Disponibile

Numero di citazioni Scopus

Non Disponibile

Ultimo Aggiornamento Citazioni

Non Disponibile

Settori ERC

Non Disponibile

Codici ASJC

Non Disponibile