Isolation of Neofusicoccum parvum from withered grapes: strain characterization, pathogenicity and its detrimental effects on passito wine
Abstract
There is scarce information on the occurrence of several fungi that infect witheredgrapes to produce passito wine. Isolation and characterization of Neofusicoccum parvumstrains and evaluation of their effects on withered grape and wine were carried out.Methods and Results: Nine isolates were phenotypically characterized by colonymorphology and genetically discriminated by molecular methods. Two representative strainswere identified as N. parvum according to phylogenetic analysis of internal transcribed spacer (ITS), and a part of translation elongation factor 1-alfa (TEF) and ?-tubulin DNA sequences.The pathogenicity of both strains on grape berries varied according to the inoculation andincubation conditions. Under withering conditions, infected berries showed browning andshriveling and some berries showed pycnidial development on the surface. The infectionaffected laccase, esterase, ?-glucosidase and tannase on grape juice as well as the content ofseveral aroma molecules on resulting wines. Strain-specific effects on wine composition werealso observed.Conclusions: N. parvum occurred in withered grapes and was able to infect grapes underwithering condition changing the aroma wine.Significance and Impact of the Study: This study reports for the first time the N. parvumisolation in fruit-drying rooms and indicates its important role on post-harvest grapeinfection.
Autore Pugliese
Tutti gli autori
-
Lorenzini M.; Cappello M.S.; Zapparoli G.
Titolo volume/Rivista
Journal of applied microbiology
Anno di pubblicazione
2015
ISSN
1365-2672
ISBN
Non Disponibile
Numero di citazioni Wos
Nessuna citazione
Ultimo Aggiornamento Citazioni
Non Disponibile
Numero di citazioni Scopus
Non Disponibile
Ultimo Aggiornamento Citazioni
Non Disponibile
Settori ERC
Non Disponibile
Codici ASJC
Non Disponibile
Condividi questo sito sui social