Isolation of Neofusicoccum parvum from withered grapes: strain characterization, pathogenicity and its detrimental effects on passito wine

Abstract

There is scarce information on the occurrence of several fungi that infect witheredgrapes to produce passito wine. Isolation and characterization of Neofusicoccum parvumstrains and evaluation of their effects on withered grape and wine were carried out.Methods and Results: Nine isolates were phenotypically characterized by colonymorphology and genetically discriminated by molecular methods. Two representative strainswere identified as N. parvum according to phylogenetic analysis of internal transcribed spacer (ITS), and a part of translation elongation factor 1-alfa (TEF) and ?-tubulin DNA sequences.The pathogenicity of both strains on grape berries varied according to the inoculation andincubation conditions. Under withering conditions, infected berries showed browning andshriveling and some berries showed pycnidial development on the surface. The infectionaffected laccase, esterase, ?-glucosidase and tannase on grape juice as well as the content ofseveral aroma molecules on resulting wines. Strain-specific effects on wine composition werealso observed.Conclusions: N. parvum occurred in withered grapes and was able to infect grapes underwithering condition changing the aroma wine.Significance and Impact of the Study: This study reports for the first time the N. parvumisolation in fruit-drying rooms and indicates its important role on post-harvest grapeinfection.


Autore Pugliese

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  • Lorenzini M.; Cappello M.S.; Zapparoli G.

Titolo volume/Rivista

Journal of applied microbiology


Anno di pubblicazione

2015

ISSN

1365-2672

ISBN

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Settori ERC

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Codici ASJC

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