Abstract

A pool of twelve cDNA sequences coding for Bowman Birk inhibitors (BBIs) was identified in the legume grass pea (Lathyrus sativus L.). The corresponding amino acid sequences showed a canonical first antitrypsin domain, predicted according to the identity of the determinant residue P-1. A more variable second binding loop was observed allowing to identify three groups based on the identity of residue P-1: two groups (Ls_BBI_1 and Ls_BBI_2) carried a second reactive site specific for chymotrypsin, while a third group (Ls_BB1_3) was predicted to inhibit elastase. A fourth variant carrying an Asp in the P-1 position of the second reactive site was identified only from genomic DNA. A phylogenetic tree constructed using grass pea BBIs with their homologs from other legume species revealed grouping based on taxonomy and on specificity of the reactive sites.

Autore Pugliese

• De Paola Domenico , Blanco Emanuela , Sonnante Gabriella

Tutti gli autori

• De Paola D.; Blanco E.; Pierri C.L.; Sonnante G.

Titolo volume/Rivista

Plant physiology and biochemistry

Anno di pubblicazione

2012

ISSN

0981-9428

ISBN

Non Disponibile

Numero di citazioni Wos

Nessuna citazione

Ultimo Aggiornamento Citazioni

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Numero di citazioni Scopus

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Ultimo Aggiornamento Citazioni

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Settori ERC

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Codici ASJC

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