Abstract

The winemaking process includes two traditional steps performed by microorganisms, alcoholic fermentation (AF) and malolactic fermentation (MLF). Even though the AF is principally carried out by the yeast Saccharomyces cerevisiae, it is not the only yeast involved in this process. Several non-Saccharomyces species, such as Hanseniaspora uvarum, have been proved to positively modify the wine chemical composition, especially contributing to the sensory characteristics of wines. The study of the Interactions between the different microbial populations present in grape must is thus important since their impact on the final quality of the wine. The main interactions occur among Saccharomyces spp. yeasts, non-Saccharomyces yeasts, and lactic acid bacteria (LAB). In the light of the rising request for autochthonous starters tailored for specific 'terroir', the objective of this work was to select the best inoculation time of autochthonous of Oenococcus oeni strains in combination with two S. cerevisiae strains and one H. uvarum strain isolated from Apulian wines. Microvinifications were performed using must derived from Apulian autochthonous grape varieties. Both, yeasts S. cerevisiae and H. uvarum, were co-inoculated and O. oeni strains were co-inoculated or sequentially inoculated during AF, when ethanol content was 2%, 4%, 6%, 8%, 10% or 12% (v/v). Ethanol formation, malic acid consumption and cell viability were monitored during the fermentative process. The obtained results indicated that the ethanol concentration at the moment of bacterial inoculation was crucial for the occurrence of a complete MLF. The co-inoculation with S. cerevisiae and H. uvarum demonstrated to be the best strategy for maintaining highest O. oeni populations and therefore for carrying out MLF in red must. This research was supported by the Apulian Region Project cod. QCBRAJ6 "Biotecnologie degli alimenti per l'innovazione e la competitività delle principali filiere regionali: estensione della conservabilità e aspetti funzionali - BIOTECA.

Autore Pugliese

• Grieco Francesco

Tutti gli autori

• V. Capozzi; C.Berbegal; A. Vernile ; F. Grieco ; G. Spano

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Anno di pubblicazione

2017

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