Integrated in vitro approaches to assess the bioaccessibility and bioavailability of silicon-biofortified leafy vegetables and preliminary effects on bone.

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Abstract

Food industries are increasingly oriented towardnew foods to improve nutritional status and/or to combat nutritionaldeficiency diseases. In this context, siliconbiofortification could be an innovative tool for obtainingnew foods with possible positive effects on bone mineralization.In this paper, an alternative and quick in vitro approachwas applied in order to evaluate the potential healthpromotingeffects of five silicon-biofortified leafy vegetables(tatsoi, mizuna, purslane, Swiss chard and chicory) on bonemineralization compared with a commercial silicon supplement.The silicon bioaccessibility and bioavailability of thefive leafy vegetables (biofortified or not) and of the supplementwere assessed by applying a protocol consisting ofin vitro gastrointestinal digestion coupled with a Caco-2 cellmodel. Silicon bioaccessibility ranged from 0.89 to 8.18 mg/Land bioavailability ranged from 111 to 206 ?g/L of Si for bothvegetables and supplement. Furthermore, the bioavailablefractions were tested on a human osteoblast cell model following the expression of type 1 collagen and alkaline phosphatase.The results obtained highlighted that the bioavailablefraction of biofortified purslane and Swiss chard improved theexpression of both osteoblast markers compared with the supplementand other vegetables. These results underline the potentiallybeneficial effect of biofortified leafy vegetables andalso indicate the usefulness of in vitro approaches for selectingthe best vegetable with positive bone effects for further in vivoresearch.

Autore Pugliese

Tutti gli autori

  • D'Imperio M.; Brunetti G. ; Gigante I. ; Serio F. ; Santamaria P. ; Cardinali A. ; Colucci S. ; Minervini F.

Titolo volume/Rivista

In vitro cellular & developmental biology. Animal

Anno di pubblicazione

2016

ISSN

1071-2690

ISBN

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Numero di citazioni Wos

Nessuna citazione

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Numero di citazioni Scopus

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Settori ERC

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Codici ASJC

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