Abstract

The gut is a possible target toward mycotoxin fumonisins (FBs) exposure. The study aims to investigatethe effects induced by FBs contaminated-corn chyme samples on functional parameters of human and ratintestine by using Ussing chamber. Fumonisins-contaminated corn and processed corn samples wereundergone to in vitro digestion process and then added to luminal side. A reduction (about 90%) of shortcircuit current (Isc lA/cm2) during exposure of human colon tissues to fumonisins-free corn chyme sampleswas observed, probably related to increased chyme osmolality. This hyperosmotic stress could drainwater towards the luminal compartment, modifying Na+ and Cl transports. The presence of FBs in cornchyme samples, independently to their concentration, did not affect significantly the Isc, probably relatedto their interference towards epithelial Na+ transport, as assessed by using a specific inhibitor (Amiloride).The rat colon tract represents a more accessible model to study FBs toxicity showing a similar functionalresponse to human. In the rat small intestine a significant reduction (about 15%) of Isc parameterduring exposure to uncontaminated or FBs contaminated corn chyme samples was observed; thereforesuch model was not suitable to assess the FBs toxicity, probably because the prevalent glucose and aminoacids electrogenic absorption overwhelmed the FBs influence on ionic transport.

Autore Pugliese

• De Girolamo Annalisa , Minervini Fiorenza , Schena Roberto

Tutti gli autori

• Minervini F.; Debellis L.; Garbetta A.; De Girolamo A.; Schena R.; Portincasa P.; Visconti A.

Titolo volume/Rivista

Food and chemical toxicology

Anno di pubblicazione

2014

ISSN

0278-6915

ISBN

Non Disponibile

Numero di citazioni Wos

Nessuna citazione

Ultimo Aggiornamento Citazioni

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Numero di citazioni Scopus

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Ultimo Aggiornamento Citazioni

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Settori ERC

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Codici ASJC

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