In vitro characterization of 6-Coumarin loaded solid lipid nanoparticles and their uptake by immunocompetent fish cells

Torna indietro

Abstract

The primary aim of the present work was to evaluate the in vitro uptake of 6-Coumarin (6COUM) loaded solid lipid nanoparticles (SLN) by two gilthead seabream (Sparus aurata L.) cell types: an established cell line (SAF-1 cells) and the primary cultures of head-kidney (HK)--the main haemopoietic organ in fish, equivalent to mammalian bone marrow--leucocytes. For this purpose, after the physicochemical characterization of SLN, the uptake by those immunocompetent fish cells was evaluated using flow cytometry and confocal microscopy. Concomitantly, the uptake of 6-COUM loaded {SLN} was compared with that achieved with 6-COUM loaded pectin microparticles (MPs), which were selected as a competitor of the delivery carriers. After {SLN} and {MP} physicochemical characterization, the results demonstrated that SAF-1 cells were able to internalize high percentages of 6-COUM {SLNs} when incubated for 4, 8 and 24&xa0;h, with the highest {SLN} concentration tested (10&xa0;?g/ml). The ability of {HK} leucocytes to internalize {SLN} was also found to vary depending on both incubation time and {SLN} concentration. The highest values of {HK} leucocytes internalizing {SLN} particles (around 16%) were detected at the maximum {SLN} concentration (20&xa0;?g/ml) at incubation times of 4 or 8&xa0;h. Conversely, {HK} leucocytes were unable to internalize {MPs} at any tested concentration and incubation time. A possible mechanism explaining the uptake into cells is proposed. The present work constitutes the first approximation to consider {SLN} as nanocarriers for delivering biologically active substances to fish.

Autore Pugliese

Tutti gli autori

  • A. Trapani; D. Mandracchia; C. Di Franco; H. Cordero; P. Morcillo; R. Comparelli; A. Cuesta; M. A. Esteban

Titolo volume/Rivista

Colloids and surfaces. B, Biointerfaces

Anno di pubblicazione

2015

ISSN

0927-7765

ISBN

Non Disponibile

Numero di citazioni Wos

Nessuna citazione

Ultimo Aggiornamento Citazioni

Non Disponibile

Numero di citazioni Scopus

Non Disponibile

Ultimo Aggiornamento Citazioni

Non Disponibile

Settori ERC

Non Disponibile

Codici ASJC

Non Disponibile