Abstract

In malignant melanoma (MM), overexpression of alfavbeta3 integrin is linked to a more metastatic phenotype. Development of anti-alfavbeta3 agents able to counteract melanoma progression would be helpful for disease treatment. A new selective ligand of alfavbeta3, RGDechi-hCit, has anti-angiogenic properties against endothelial cells in angiogenesis animal models. Aim of this study was to evaluate the in vitro effects of the RGDechi-hCit peptide on MM cell lines. Material and Methods: Cytofluorimetric analysis characterized cell surface expression of alfavbeta3 integrin on seven MM cell lines. Cell proliferation, adhesion, and migration assays were carried out using the alfavbeta3-antagonist RGDechi-hCit. Results: Proliferation was not significantly inhibited by RGDechi-hCit, though striking morphological changes were detected in MM cell lines highly expressing alfavbeta3. Conversely, assays on fibronectin-coated plates showed a significant RGDechi-hCit dose-dependent inhibitory effect on both adhesion and migration. Conclusions: Data demonstrate anti-adhesion and anti-migration, but not anti-proliferative, activities of RGDechi-hCit against MM cells.

Autore Pugliese

• Saviano Michele

Tutti gli autori

• Pisano M.; de Paola I.; Nieddu V.; Sassu I.; Cossu S.; Galleri G.; Del Gatto A.; Budroni M.; Cossu A.; Saviano M.; Palmieri G.; Zaccaro L.; Rozzo C.

Titolo volume/Rivista

Anticancer research

Anno di pubblicazione

2013

ISSN

0250-7005

ISBN

Non Disponibile

Numero di citazioni Wos

Nessuna citazione

Ultimo Aggiornamento Citazioni

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Numero di citazioni Scopus

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Ultimo Aggiornamento Citazioni

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Settori ERC

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Codici ASJC

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