How a Small Double-Stranded Trick Can Mislead Sanger Sequencing.

Abstract

Notwithstanding the arrival of "third-generation sequencing," Sanger sequencing, developed in 1980, is still the most accurate and used method for sequencing, although on a smaller scale. It is a powerful resource for studying sequences and discovering polymorphisms and genes, as well as regulatory elements. There has already been described a wide range of possible problems with this very sensitive and accurate technology. Here, we show that a specific event, related to genomes rich in repetitive sequences, can mislead operators working with Sanger sequencing.


Autore Pugliese

Tutti gli autori

  • Curci P.L.; Sonnante G.

Titolo volume/Rivista

Journal of biomolecular techniques


Anno di pubblicazione

2015

ISSN

1524-0215

ISBN

Non Disponibile


Numero di citazioni Wos

Nessuna citazione

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Settori ERC

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Codici ASJC

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