Abstract

Early diagnosis of plant virus infections before the disease symptoms appearance may represent a significant benefit in limiting disease spread by a prompt application of appropriate containment steps. We propose a label-free procedure applied on a device structure where the electrical signal transduction is evaluated via impedance spectroscopy techniques. The device consists of a droplet suspension embedding two representative purified plant viruses i.e.,Tomato mosaic virus and Turnip yellow mosaic virus,put in contact with a highly hydrophobic plasma textured silicon surface. Results show a high sensitivity of the system towards the virus particles with an interestingly low detection limit,from tens to hundreds of attomolar corresponding to pg/mL of sap,which refers,in the infection time-scale,to a concentration of virus particles in still-symptomless plants. Such a threshold limit,together with an envisaged engineering of an easily manageable device,compared to more sophisticated apparatuses,may contribute in simplifying the in-field plant virus diagnostics.

Autore Pugliese

• Ambrico Marianna , Ambrico Paolo Francesco , Cicco Stefania Roberta , Minafra Angelantonio , De Stradis Angelo

Tutti gli autori

• Ambrico M.; Ambrico P.F.; Minafra A.; De Stradis A.; Vona D.; Cicco S.R.; Palumbo F.; Favia P.; Ligonzo T.

Titolo volume/Rivista

Sensors

Anno di pubblicazione

2016

ISSN

1424-8220

ISBN

Non Disponibile

Numero di citazioni Wos

Nessuna citazione

Ultimo Aggiornamento Citazioni

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Numero di citazioni Scopus

Non Disponibile

Ultimo Aggiornamento Citazioni

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Settori ERC

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Codici ASJC

Non Disponibile