Abstract

The present investigation was aimed to study theuptake of glucose capped silver nanoparticles (AgNPs-G) byhuman epithelioid cervix carcinoma (HeLa) cells and thenanoparticles effect on cell cycle progression. Cells were exposedto two different amounts (2x103 and 2x104 NPs/cell) of AgNPs-G(average size 10 nm) for different times (15 and 30 minutes, 1, 3,6, 12, 18 and 24 hrs). The uptake of AgNPs by HeLa cells wasevaluated by using Graphite Furnace-Atomic AbsorptionSpectrometry (GF-AAS) and Scanning Electron Microscopy-Energy Dispersive X-ray (SEM-EDX) analysis. Cell cycle wasinvestigated by Fluorescence Activated Cell Sorting (FACS)analysis. AgNPs-G were abundantly taken up by HeLa cellswithin 2 h of treatment and induced cytotoxicity in a NPsamount- and incubation time- dependent manner. The treatmentalso determined a AgNPs-G concentration- and time-dependent Sand G2/M arrest. The possible influence of the cell cycle oncellular uptake of AgNPs-G needs, however, to be furtherinvestigated since the dose of internalized nanoparticles in eachcell could vary as the cell advances through the cell cycle.

Autore Pugliese

• Casciaro Sergio

Tutti gli autori

• E. Panzarini; S. Mariano; C. Vergallo; G.M. Fimia; L. Dini; F. Mura; M. Rossi; A. Serra; S. Casciaro.

Titolo volume/Rivista

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Anno di pubblicazione

2015

ISSN

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ISBN

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Numero di citazioni Wos

Nessuna citazione

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Numero di citazioni Scopus

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Ultimo Aggiornamento Citazioni

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Settori ERC

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Codici ASJC

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