Abstract

Exploiting the biodiversity of crops and their wild relatives is fundamental for maintaining andincreasing food security. The species Cynara cardunculus includes three taxa: the globe artichoke,one of the most important Mediterranean vegetables, the leafy cardoon, and the wildcardoon. In this study, genotyping by sequencing (GBS) was successfully applied to revealthousands of polymorphisms in a C. cardunculus germplasm collection, including 65 globeartichoke, 9 leafy cardoon, and 21 wild cardoon samples. The collection showed a strong populationstructure at K = 2, separating the globe artichoke from the leafy and wild cardoon. Athigher K values, further substructures were observed, in which the wild cardoon was separatedfrom the leafy cardoon, and the latter included the Spanish wild cardoons, while the wildsample from Portugal was admixed. Moreover, subpopulations within the globe artichoke setwere highlighted. Structure analysis restricted to the globe artichoke dataset pointed outgenetic differentiation between the ?Catanesi? typology and all the other samples (K = 2). Athigher values of K, the separation of the ?Catanesi? group still held true, and green headedlandraces from Apulia region, Italy (?Green Apulian?) formed a distinct subpopulation. ?Romaneschi?artichoke types fell in a variable group with admixed samples, indicating that theyshould not be considered as a genetically uniform typology. The results of principal componentanalysis and Neighbor-Joining hierarchical clustering were consistent with structureresults, and in addition provided a measure of genetic relationships among individual genotypes.Both analyses attributed the wild material from Spain and Portugal to the cultivated cardoongroup, supporting the idea that this might be indeed a feral form of the leafy cardoon.Different reproductive habit and possibly selective pressure led to a slower LD decay in artichokecompared to cardoon. Genotyping by sequencing has proven a reliable methodology toobtain valuable SNPs and assess population genetics in C. cardunculus

Autore Pugliese

• Blanco Emanuela , Sonnante Gabriella

Tutti gli autori

• Pavan S.; Curci P.L.; Zuluaga D.L.; Blanco E.; Sonnante G.

Titolo volume/Rivista

PloS one

Anno di pubblicazione

2018

ISSN

1932-6203

ISBN

Non Disponibile

Numero di citazioni Wos

Nessuna citazione

Ultimo Aggiornamento Citazioni

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Numero di citazioni Scopus

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Ultimo Aggiornamento Citazioni

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Settori ERC

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Codici ASJC

Non Disponibile