Abstract

In plants, RNA silencing is a surveillance mechanism against invading viruses. It involves the production of virus-derived small interfering RNAs (vsiRNAs), which guide the RNA-induced silencing complex (RISC) to inactivate viruses. vsiRNAs may also promote the silencing of host mRNAs in a sequence-specificmanner. In this work, vsiRNAs derived from two grapevineinfecting viruses (Grapevine fleck virus and Grapevine rupestris stem pitting-associated virus) were selected from cDNA libraries of short RNAs and were cross-referenced with the remnants of both cleaved host transcripts and viral RNAs from a degradome dataset. We identified dozens of host transcripts targeted byvsiRNAs. Among them, several encode putative proteins involved in ribosome biogenesis and in biotic and abiotic stresses. Moreover, we identified vsiRNAs which explain the cleavage sites in viral genomes. A consistent fraction of vsiRNAs did not apparentlyaccount for cleavage, suggesting that only a low percentage of vsiRNAs are involved in the antiviral response.

Autore Pugliese

• Pantaleo Vitantonio

Tutti gli autori

• Miozzi L.; Gambino G.; Burgyan J.; Pantaleo V.

Titolo volume/Rivista

Molecular plant pathology

Anno di pubblicazione

2013

ISSN

1464-6722

ISBN

Non Disponibile

Numero di citazioni Wos

Nessuna citazione

Ultimo Aggiornamento Citazioni

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Numero di citazioni Scopus

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Ultimo Aggiornamento Citazioni

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Settori ERC

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Codici ASJC

Non Disponibile