Abstract

Phenolic acids are major components of cell walls in wheat and have important implicationson human health as antioxidants with anti-tumor activity. Understanding the genetic basis ofphenolic acids content and composition of wheat grain, and identifying associated markers providethe basis for improving wheat quality by molecular breeding. The objective of this study was: a) toidentify phenolic acids genes in wheat by exploiting genomic resources and SNPs detected withinthe coding sequences of candidate genes; and b) to identify chromosomal regions associated tosingle phenolic acids and total soluble phenolic compounds by genome-wide association study(GWAS) and candidate gene approaches using a tetraploid wheat collection coupled with the 90KiSelect SNP genotyping array. A set of candidate genes involved in the biosynthesis ofhydroxycinnamic acid derivatives (p-coumaric acid, ferulic acid and sinapic acid) and in the totalsoluble phenolics have been identified in wheat by comparative genomics. Single nucleotidepolymorphisms (SNPs) found in the coding sequences of six candidate genes (PAL1, PAL2, C4H,C3H, COMT1 and COMT2) allowed to determine their chromosomal location and accurate mapposition on two reference consensus linkage maps. The genome-wide association study based ongenotyping a tetraploid wheat collection with 81,587 gene-associated SNPs revealed severalsignificant marker-trait associations, identifying 22 quantitative trait loci (QTLs) associated withindividual phenolic acids and total soluble phenol compounds distributed on almost all durumwheat chromosomes. A QTL for p-coumaric acid was found to be coincident with the candidategene phenylalanine ammonia-lyase (PAL2) on chromosome arm 2AL. The availability of perfect,candidate gene-based markers can allow elucidating the mechanism of phenol acids accumulation inthe wheat kernels and exploiting the genetic variability of phenol acids content for the nutritionalimprovement of wheat end-products.

Autore Pugliese

• Laddomada Barbara , Mita Giovanni

Tutti gli autori

• NIGRO D.; LADDOMADA B.; MITA G.; ZACHEO S.; SIMEONE R.; GADALETA A.; PASQUALONE A.; BLANCO A.

Titolo volume/Rivista

Non Disponibile

Anno di pubblicazione

2016

ISSN

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ISBN

978-88-904570-6-7

Numero di citazioni Wos

Nessuna citazione

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Numero di citazioni Scopus

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Settori ERC

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Codici ASJC

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