Abstract

The Fusarium incarnatum-equiseti species complex (FIESC) includes mycotoxigenicspecies associated with several diseases of cereals and other crops. Althoughthese species are considered as moderately aggressive, they are able to producemultiple mycotoxins, including beauvericin, zearalenone, equisetin,fusarochromanone, butenolide as well as the trichothecenes DAS, MAS, FUS-X,DON, NIV, and scirpentriol. Thus, members of FIESC are potential contributors tomycotoxin contamination of cereals. FIESC includes high levels of crypticspeciation as most species within the complex cannot be distinguished from oneanother by morphological traits. However, a previous DNA-based analysis ofhuman isolates from the US resolved FIESC into 28 phylogenetically distinctlineages, or multilocus sequence types (MLSTs). Here, we investigated thephylogenetic diversity of 69 FIESC isolates recovered from cereals grown in Europeand North America by comparison to the previously described MLSTs. Inphylogenetic analyses of the four housekeeping genes EF-1a, RPB2, CaM andTUB2, 4 isolates were resolved within the F. incarnatum clade of FIESC, and all otherisolates were resolved within the F. equiseti clade. However, 8 isolates wereresolved into a lineage that is distinct from all previously described MLSTs,suggesting that they constitute novel MLST within FIESC. Phylogenomic analysis of12 isolates, representing one novel and 11 previously described and MLSTs, inferreda phylogeny that was consistent with but more highly resolved than the phylogenyinferred from fourgenes. Comparative analysis of the genome sequences revealedvariation in distribution of mycotoxin biosynthetic gene clusters. For example, thetrichothecene cluster is present in all nine genomes, whereas the fusarin andzearalenone clusters are present in only three and four genomes respectively.These data indicate that different FIESC MLSTs vary in their genetic potential toproduce and contaminate cereal crops with different mycotoxins.

Autore Pugliese

• Susca Antonia , Moretti Antonio , Logrieco Antonio Francesco

Tutti gli autori

• Villani A.; Proctor R.H.; Brown D.W.; Ward T.J.; Logrieco A.;Moretti A.; Susca A.

Titolo volume/Rivista

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Anno di pubblicazione

2015

ISSN

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ISBN

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Numero di citazioni Wos

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Settori ERC

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Codici ASJC

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