Formation of Self-Assembled Triple-Layered Rotavirus-Like Particles (tlRLPs) by Constitutive Co-Expression of VP2, VP6, and VP7 in Stably Transfected High-Five Insect Cell Lines

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Abstract

In this study, stable high-five insect cell line constitutively expressing rotavirus (RV) VP2 was co-transfected with VP6 and VP7-recombinant plasmids. The presence of RV proteins in stably transfected high-five cells was verified by molecular and protein analyses. To yield self-assembled triple-layered RV-like particles (tlRLPs), a stable insect high-five cell line was generated to produce RV VP6 and VP7 besides VP2. Self-assembled tlRLPs were observed by transmission electron microscopy (TEM), and enzyme-linked immunosorbent assay (ELISA) was used to assess their antigenicity in vivo. The results suggest that the stable transfected high-five cells are able to generate tlRLPs with the efficient antigenicity. J. Med. Virol. 87: 102-111, 2015. (c) 2014 Wiley Periodicals, Inc.

Autore Pugliese

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  • Shoja Z.; Tagliamonte M.; Jalilvand S.; Mollaei-Kandelous Y.; De Stradis A.; Tornesello M.L.; Buonaguro F.M.; Buonaguro L.

Titolo volume/Rivista

Journal of medical virology

Anno di pubblicazione

2015

ISSN

0146-6615

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