Abstract

In order to evaluate the incidence of virus diseases in apple, pear and quince in Lebanon, a large-scale survey was carried out in the main pome fruit-producing areas. To this aim, the most important commercial orchards and nurseries in the ountry were tested by a combination of biological (woody indexing and mechanical inoculation), serological (ELISA) and molecular (RT-PCR) assays in order to detect the following viruses: Apple chlorotic leaf spot virus (ACLSV), Apple stem pitting virus (ASPV), Apple stem grooving virus (ASGV), and Apple mosaic virus (ApMV). A total of 968 samples (713 apples, 175 pears and 80 quinces) were tested by DAS-ELISA, revealing that ACLSV, ASPV and ASGV were prevalent in apple trees, with an average rate of infection with at least one of the viruses corresponding to 27%. The incidence of the same viruses in pear samples was 1 % whereas no virus was detected in quince. ACLSV was the most predominant virus (16%) followed by ASPV (13 %) and ASGV (2.5%). In contrast, ApMV was not detected in any of the tested samples. RT-PCR assays performed on a fraction of 100 samples, confirmed the DAS-ELISA results, although additional positive samples were identified, showing a higher sensitivity of the former with respect to the latter detection method. The implications of our study for improving the phytosanitary status of pome fruit trees in Lebanon will be discussed.

Autore Pugliese

• Di Serio Francesco

Tutti gli autori

• Nassar E.; Choueiri E.; Minoia S.; Di Serio F.; Djelouah K.

Titolo volume/Rivista

Petria

Anno di pubblicazione

2012

ISSN

1120-7698

ISBN

Non Disponibile

Numero di citazioni Wos

Nessuna citazione

Ultimo Aggiornamento Citazioni

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Numero di citazioni Scopus

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Ultimo Aggiornamento Citazioni

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Settori ERC

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Codici ASJC

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