Abstract

During October 2015, severe symptoms of collar rot wereobserved in a commercial field of two month-old cauliflower(Brassica oleracea L. var. botrytis) in BAT province (Apulia,southern Italy). Among several cultivars grown in the samefield (6 ha), only cultivar Tipoff F1 (Bejo) showed diseasesymptoms with ca. 5% of plants affected. First symptoms appearedon collar tissues and consisted of wide water-soakedlesions which progressively darkened, rotted and extendedto the midrib of basal leaves.Pythium-like colonies were consistently isolated fromdecayed plants on potato dextrose agar (PDA), and onerepresentative isolate, designated DiSSPA P8, showed morphologicalcharacters consistent with those described by vander Plaats-Niterink (1981) for the type specimen CBS 398.51of Pythium ultimum Trow var. ultimum, recently renamedGlobisporangium ultimum (Trow) Uzuhashi, Tojo & Kakish.(Uzuhashi et al., 2010).The coxII gene as well as ITS and D1/D2 regions of rDNAwere sequenced and deposited in GenBank under accessionNos. KY753869, KY392755 and KY753868, respectively. Oncethese sequences were included in the phylogenetic trees constructedby Uzuhashi et al. (2010), DiSSPA P8 clustered withG. ultimum strain UZ056 with a 99% sequence identity to thecorresponding sequences from this isolate.Furthermore, the representative isolate induced collarrot symptoms, similar to those observed in the field, on sixweek-old cauliflower plants cv. Tipoff F1 after two days at26°C from inoculation with colonized PDA discs on superficiallywounded stem.To the best of our knowledge, this is the first report ofcollar rot caused by G. ultimum on cauliflower in Italy.

Autore Pugliese

• Bubici Giovanni Nicola

Tutti gli autori

• Cariddi C.; Miacola C.; Prigigallo M.I.; Bubici G.

Titolo volume/Rivista

Journal of plant pathology

Anno di pubblicazione

2017

ISSN

1125-4653

ISBN

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Numero di citazioni Wos

Nessuna citazione

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Settori ERC

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Codici ASJC

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