Abstract

Cadmium is a highly toxic heavy metal with negative effects on oocyte fertilization. The aim of this study was to analyse whether cadmium-induced impairment of fertilization is caused by mitochondria dysfunction and oxidative stress in the cumulus-oocyte complex (COC). Preliminarily, 19 trace element levels were measured in ovaries from juvenile and adult ewes and age-related cadmium ovarian bioaccumulation at nanomolar concentrations was found. COCs from juvenile and adult ewes, exposed during in vitro maturation to 1 nM or 100 nM CdCl2, and subjected to in vitro fertilization showed significantly lower fertilization rates in exposed COCs compared with controls. In vitro matured exposed and control COCs underwent confocal microscopy analysis of mitochondria activity and reactive oxygen species (ROS) levels and lipid peroxidation (LPO) assay at cumulus cell and oocyte level. In both age groups, cadmium at nanomolar concentrations induced cumulus-oocyte mitochondria over-activity and oxidative damage which were related to impaired oocyte fertilization. (C) 2017 Elsevier Inc. All rights reserved.

Autore Pugliese

• Minervini Fiorenza

Tutti gli autori

• Martino N. A.; Marzano G.; Mangiacotti M.; Miedico O.; Sardanelli A. M.; Gnoni A.; Lacalandra G. M.; Chiaravalle A. E.; Ciani E.; Bogliolo L.; Minervini F.; Pizzi F.; Dell'Aquila M. E.

Titolo volume/Rivista

Reproductive toxicology

Anno di pubblicazione

2017

ISSN

0890-6238

ISBN

Non Disponibile

Numero di citazioni Wos

Nessuna citazione

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Numero di citazioni Scopus

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Settori ERC

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Codici ASJC

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