Exploiting a new glycerol-based copolymer as a route to wound healing: Synthesis, characterization and biocompatibility assessment

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Abstract

tThe use of biocompatible materials based on naturally derived monomers plays a key role in pharma-ceutical and cosmetic industries. In this paper we describe the synthesis of a new low molecular weightcopolymer, based on glycerol and l-tartaric acid, useful to develop biocompatible dermal patches withdrug delivery properties. The copolymer's chemical composition was assessed by FT-IR (Fourier trans-form infrared spectroscopy),1H NMR (1H Nuclear Magnetic Resonance) and XPS (X-ray photoelectronspectroscopy), while its molecular weight distribution was estimated by SEC (size exclusion chromatog-raphy). Copolymer thermal properties were studied by TGA (thermogravimetric analysis). Biologicalevaluations by MTT assay and SEM (scanning electron microscopy) observations performed with murinefibroblasts and human keratinocytes (HaCaT) revealed a good compatibility of the proposed copolymer.Ciprofloxacin was selected as model drug and its release was evaluated by HPLC (high performance liquidchromatography), showing that the new copolymer supplied promising results as drug delivery systemfor wound healing applications. Furthermore, investigations on Skin-Mesenchymal stem cells (S-MSCs)behaviour and gene expression showed that the copolymer and its combination with ciprofloxacin didnot affect their stemness. In this regard, the fabrication of dermal patches with new, low cost materials forlocal treatment of skin infections represents an attractive strategy in order to bypass the worrying sideeffects of systemic antibiotic therapy. Overall, the performed physico-chemical characterization, drugrelease test and biological evaluations showed that this new copolymer could be a promising tool for thein situ delivery of bioactive molecules during skin lesions treatment.

Autore Pugliese

Tutti gli autori

  • De Giglio E.; Bonifacio M.A.; Cometa S.; Vona D.; Mattioli-Belmonte M.; Dicarlo M.; Ceci E.; Fino V.; Cicco S.R.; Farinola G.M.

Titolo volume/Rivista

Colloids and surfaces. B, Biointerfaces

Anno di pubblicazione

2015

ISSN

0927-7765

ISBN

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Numero di citazioni Wos

Nessuna citazione

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Numero di citazioni Scopus

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Settori ERC

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Codici ASJC

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