Abstract

The quarantine bacterium Xylella fastidiosa (Xf) is responsible for diseases of a wide range of cultivated and wild plants. Few ef- forts have been made to investigate the potential use of endophytic symbionts on the disease phenotype of Xf-infected plants. The aim of our study was to evaluate if Paraburkholderia phytofirmans PsJN strain, a plant growth-promoting rhizobacterium, whose beneficial effects in the reduction of symptom severity caused by Xf in grape- vine affected by Pierce's Disease have recently been proven, may play a role as biocontrol agent against Xf CoDiRO strain, the agent of a severe disease of olives in Apulia (southern Italy). Greenhouse trials are being conducted to test the ability of P. phytofirmans to colonise xylem vessels of olive, Nicotiana benthamiana and oleander, following inoculation of bacterial suspensions by needle puncture and root dipping. A conventional PCR assay for detection of P. phytofirmans movement in plants has been developed to be used in combination with plate isolation and a qPCR specific assay. Preliminary results showed that needle-inoculated bacterial cells were detectable in the leaf petioles of the three hosts, away from the inoculation site. Root dipping proved successful in infecting in vitro-cultured olive plantlets. Double-infection assays, currently underway, will prove if P. phytofirmans PsJN shows a beneficial interaction with Xf CoDiRO.

Autore Pugliese

• Morelli Massimiliano , Saldarelli Pasquale

Tutti gli autori

• M. Morelli; P. Saldarelli

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Anno di pubblicazione

2017

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ISBN

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Settori ERC

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