Abstract

Alginate micro beads containing Lactobacillus kefiri (the principal bacteria present in the kefir probiotic drink)were produced by a novel technique based on dual aerosols spaying of alginate based solution and CaCl2 ascross linking agent. Carboxymethylcellulose (CMC) has been also added to the alginate in order to change thephysic-chemical properties (viscosity and permeability) of the microbeads. Calcium alginate and CMC are biopolymersthat can be used for developing oral drug-delivery systems. These biopolymers have been reportedto show a pH-dependent swelling behaviour. Calciumalginate and CMC have also been known to possess an excellentmucoadhesive property. The loaded microbeads have been characterized in terms of morphology, chemicalcomposition and stability in different conditions mimicking the gastric environment.In this study, we demonstrate the feasibility of a continuous fabrication of alginate microbeads in a range of50-70 ?m size, encapsulating L. kefiri as active ingredient. The technique involves the use of a double aerosols ofalginate based solution and CaCl2 as crosslinking agent. Moreover, the encapsulation process was proved to beeffective and not detrimental to bacteria viability. At the same time, it was verified the protective efficacy ofthe microcapsules against the gastric environment using both SGF pH 1.2 (fasted state) and pH 2.2 (feed state).

Autore Pugliese

• Cappello Maria Stella

Tutti gli autori

• Demitri C.; Lamanna L.; De Benedetto E.; Damiano F.; Cappello M.S.; Siculella L.; Sannino A.

Titolo volume/Rivista

Materials science & engineering. C, Biomimetic materials, sensors and systems

Anno di pubblicazione

2017

ISSN

0928-4931

ISBN

Non Disponibile

Numero di citazioni Wos

Nessuna citazione

Ultimo Aggiornamento Citazioni

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Numero di citazioni Scopus

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Ultimo Aggiornamento Citazioni

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Settori ERC

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Codici ASJC

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