Early and late transcriptome changes in a tomato cultivar carrying Sw-5 resistance gene upon infection by a resistance-breaking strain of Tomato spotted wilt virus

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Abstract

We analyzed the transcriptome (RNA-Seq) of leaf samples collected from a field crop of tomato cv. Docet (Sw5 resistance gene) in Apulia, southern Italy, with different symptom severity and accumulation levels of a resistance-breaking strain of Tomato spotted wilt virus (TSWV). Four groups of samples were assumed to be different stages of plant tissue colonization by the virus: plants without symptoms anda virus titre (group A) or 1 ×?102 TSWV reads per million (rpm; B), and plants with symptoms and 1 ×?104 rpm (C) or 2 ×?105 rpm (D). Transcriptome sequencing revealed that plant response to TSWV infection is profoundly related to its accumulation level in the tissues. At an early stage of infection (B vs. A comparison), genes related to photosystem I were down-regulated, and oxidoreductase activityincreased. Considerable virus colonization (C vs. B) activated defense-related mechanisms such as cell surface receptor signalling, phenylpropanoid biosynthesis and transcription factor activity. In contrast, photosynthesis, transmembrane transporter activity, and biosynthesis of monosaccharides and peptides were down-regulated. This scenario increased at an advanced stage of colonization (D vs.C), with attenuation of response to stimuli (e.g., surface receptor signaling and protein kinase activity) and an increase of catalytic activities such as ubiquitin- protein transferase and ribonuclease. TSWV infection constantly injured tomato cell metabolism(e.g., photosynthesis, monosaccharide and peptide biosynthesis, ion transporter activity) while plant defense (e.g., cell surface receptor signaling, phenylpropanoid pathway), clearly ineffective in such compatible plant-virus interaction, occurred late and disappeared soon after.

Autore Pugliese

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  • Bubici G.; Cillo F.; Prigigallo M.I.; Monfreda R.

Titolo volume/Rivista

Phytopathologia Mediterranea

Anno di pubblicazione

2017

ISSN

1593-2095

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